id: 23468
Title: The effectiveness of various methods for determining the viability of A. simplex larvae in fish products
Authors: Shevchuk T. V.
Keywords: A. simplex larvae, methods, viability, fishery products, efficiency
Date of publication: 2020-02-07 15:17:18
Last changes: 2020-02-07 15:17:18
Year of publication: 2019
Summary: The article presents the results of a comparative analysis of various methods for determining the viability of A. simplex larvae in fish products. The method of activating larvae in the physiological solution was the fastest, affordable and less expensive. The method of incubation in artificial gastric juice received more unsatisfactory estimates. Its execution is possible only in the presence of reagents and a thermostat. During parasitological studies, the severity of the A. simplex invasion in fish products was 82,7%, the intensity of the invasion was 3-10 units, the invasion index was 7.8 units. All A. simplex larvae that have been detected in fish have been gradually tested by different viability methods. It has been experimentally established that different methods for determining the viability of A. simplex larvae have different accuracy. The least accurate methods have been found to activate live larvae in a physiological solution with mechanical and electrical stimulation. More precise was the method for determining the viability of A. simplex larvae using methylene blue. During the painting live parasites retained their natural color, and the dead - acquired a blue color. The disadvantage of this method is the duration of the experiment and the ambiguity of the results of the study. Separate larvae after removal from capsules were painted differently. Therefore, the interpretation of research results was complicated. In this method, the largest number of live parasites were detected in a group of fish products. In addition, A. simplex live larvae were detected by this method, not only in the abdominal cavity, but also in the wall of the abdominal cavity and muscles. The most accurate methods were electrical activation, color and digestion in artificial gastric juice. We were able to detect A. simplex live larvae that were not identified in saline solution, even after exposure to a thermostat and mechanical stimulation, by these methods. In this case, the accuracy of the method of electrical irritation was 4.35% lower than the color of the indicator and the use of artificial gastric juice. In the future, it is planned to investigate the effectiveness of the method of availability and determination of the viability of A. simplex larvae by biological resonance method.
URI: http://socrates.vsau.org/repository/getfile.php/23468.pdf
Publication type: Статті у наукових фахових виданнях України (Copernicus та інші)
Publication: Водні біоресурси та аквакультура. - 2019. - № 2. - С. 186-200.
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Published by: Адміністратор
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